Journal: Molecules

Article Title: The Structure–Antimicrobial Activity Relationships of a Promising Class of the Compounds Containing the N-Arylpiperazine Scaffold

doi: 10.3390/molecules21101274

Figure Lengend Snippet: Figure 4. The R-substituted phenylcarbamic acid derivatives screened in vitro against M. tuberculosis H37Rv, M. kansasii CNCTC My 235/80, M. avium CNCTC My 330/88 and M. kansasii 6509/96.

Article Snippet: The molecules were screened against Mycobacterium avium subsp. paratuberculosis CIT03, M. smegmatis ATCC 700084, M. kansasii DSM 44162, M. marinum CAMP 5644, Staphylococcus aureus ATCC 29213, methicillin-resistant S. aureus 63718, Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212, Candida albicans CCM 8261, C. parapsilosis CCM 8260 and C. krusei CCM 8271, respectively, by standardized microdilution methods.

Techniques: In Vitro

Journal: Molecules

Article Title: The Structure–Antimicrobial Activity Relationships of a Promising Class of the Compounds Containing the N-Arylpiperazine Scaffold

doi: 10.3390/molecules21101274

Figure Lengend Snippet: Figure 5. Chemical structure of 2-hydroxy-3-[(2-aryloxyethyl)amino]propyl-4-[(alkoxycarbonyl)amino]benzoates, which were efficient in vitro against M. avium subsp. paratuberculosis CIT03 strain.

Article Snippet: The molecules were screened against Mycobacterium avium subsp. paratuberculosis CIT03, M. smegmatis ATCC 700084, M. kansasii DSM 44162, M. marinum CAMP 5644, Staphylococcus aureus ATCC 29213, methicillin-resistant S. aureus 63718, Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212, Candida albicans CCM 8261, C. parapsilosis CCM 8260 and C. krusei CCM 8271, respectively, by standardized microdilution methods.

Techniques: In Vitro

Journal: International journal of mycobacteriology

Article Title: Molecular epidemiology of Mycobacterium avium subspecies paratuberculosis in ruminants in different parts of India.

doi: 10.1016/j.ijmyco.2015.11.003

Figure Lengend Snippet: Fig. 1 – IS900 PCR. Lane M, marker; lane 1, C-132; lane 2, C1A; lane 3, 163A; lane 4, CS2028; lane 5, K10 MAP. Note: MAP = Mycobacterium avium subspecies paratuberculosis; PCR = polymerase chain reaction.

Article Snippet: The cattle ‘C’ type was detected only in seven cattle isolates from the Bareilly region (North India) and produced four bands by PCR-REA (67 bp, 218 bp, 285 bp, and 323 bp). ogy of Mycobacterium avium subspecies paratuberculosis in ruminants /10.1016/j.ijmyco.2015.11.003 All MAP reference strains (K10, ATCC 19698, and 316F) were ‘C’ type, while M. avium (M120/04 UK) showed an Maa-type REA pattern as expected.

Techniques: Marker, Polymerase Chain Reaction

Journal: International journal of mycobacteriology

Article Title: Molecular epidemiology of Mycobacterium avium subspecies paratuberculosis in ruminants in different parts of India.

doi: 10.1016/j.ijmyco.2015.11.003

Figure Lengend Snippet: Fig. 2 – IS1311 gene PCR. Lane M, marker; lane 1, C-132; lane 2, C1A; lane 3, 163A; lane 4, CS2028; lane 5, C16; lane 6, H615; lane 7, K10 MAP. Note: MAP = Mycobacterium avium subspecies paratuberculosis; PCR = polymerase chain reaction.

Article Snippet: The cattle ‘C’ type was detected only in seven cattle isolates from the Bareilly region (North India) and produced four bands by PCR-REA (67 bp, 218 bp, 285 bp, and 323 bp). ogy of Mycobacterium avium subspecies paratuberculosis in ruminants /10.1016/j.ijmyco.2015.11.003 All MAP reference strains (K10, ATCC 19698, and 316F) were ‘C’ type, while M. avium (M120/04 UK) showed an Maa-type REA pattern as expected.

Techniques: Marker, Polymerase Chain Reaction

Journal: International journal of mycobacteriology

Article Title: Molecular epidemiology of Mycobacterium avium subspecies paratuberculosis in ruminants in different parts of India.

doi: 10.1016/j.ijmyco.2015.11.003

Figure Lengend Snippet: Fig. 3 – PCR-REA analysis of MAP isolates. Lane M1 and M2, markers; lane 1, C-139 (B type); lane 2, C-12A (B type); lane 3, 316FMTCC (C type); lane 4, C-148A (C type); lane 5, 1168A (B type); lane 6, Mycobacterium bovis (no band pattern). Note: MAP = Mycobacterium avium subspecies paratuberculosis; PCR = polymerase chain reaction; REA = restriction endonuclease analysis.

Article Snippet: The cattle ‘C’ type was detected only in seven cattle isolates from the Bareilly region (North India) and produced four bands by PCR-REA (67 bp, 218 bp, 285 bp, and 323 bp). ogy of Mycobacterium avium subspecies paratuberculosis in ruminants /10.1016/j.ijmyco.2015.11.003 All MAP reference strains (K10, ATCC 19698, and 316F) were ‘C’ type, while M. avium (M120/04 UK) showed an Maa-type REA pattern as expected.

Techniques: Polymerase Chain Reaction

Journal: International journal of mycobacteriology

Article Title: Molecular epidemiology of Mycobacterium avium subspecies paratuberculosis in ruminants in different parts of India.

doi: 10.1016/j.ijmyco.2015.11.003

Figure Lengend Snippet: Fig. 4 – PCR-REA analysis of MAP isolates. Lane M1 and M2, marker; lane 1, 163A (B type); lane 2, C-132 (B type); lane3, CS2280 (B type); lane 4, CS1700 (B type); lane 5, C 93 (B type); lane 6, C260 (B type). Note: MAP = Mycobacterium avium subspecies paratuberculosis; PCR = polymerase chain reaction; REA = restriction endonuclease analysis.

Article Snippet: The cattle ‘C’ type was detected only in seven cattle isolates from the Bareilly region (North India) and produced four bands by PCR-REA (67 bp, 218 bp, 285 bp, and 323 bp). ogy of Mycobacterium avium subspecies paratuberculosis in ruminants /10.1016/j.ijmyco.2015.11.003 All MAP reference strains (K10, ATCC 19698, and 316F) were ‘C’ type, while M. avium (M120/04 UK) showed an Maa-type REA pattern as expected.

Techniques: Marker, Polymerase Chain Reaction

Journal: Applied and Environmental Microbiology

Article Title: Development of an F57 Sequence-Based Real-Time PCR Assay for Detection of Mycobacterium avium subsp. paratuberculosis in Milk

doi: 10.1128/aem.71.10.5957-5968.2005

Figure Lengend Snippet: FIG. 1. Dynamics of F57 sequence and IC template coamplification under optimized LC-PCR assay conditions: graphic plot from the light cycler. The data show the results for coamplification of the F57 sequence standard dilutions and a constant amount of the IC template (2,000 copies) in the LC-PCR assay. The large graph shows M. avium subsp. paratuberculosis F57 amplification as monitored in the LC-Red 705 channel, and the inset shows IC template amplification as monitored in the LC-Red 640 channel. The inset shows that the amount of IC template used is large enough to effectively monitor amplification in the presence of small amounts of the M. avium subsp. paratuberculosis F57 target (0 to 2 103

Article Snippet: a Replicates containing different amounts (140 fg to 10 fg) of M. avium subsp. paratuberculosis genomic DNA isolated from a pure culture of the M. avium subsp. paratuberculosis reference strain (ATCC 6783) were subjected to LC-PCR amplification as outlined in Materials and Methods.

Techniques: Sequencing

Journal: Applied and Environmental Microbiology

Article Title: Development of an F57 Sequence-Based Real-Time PCR Assay for Detection of Mycobacterium avium subsp. paratuberculosis in Milk

doi: 10.1128/aem.71.10.5957-5968.2005

Figure Lengend Snippet: FIG. 2. Evaluation of the specificity of the M. avium subsp. paratuberculosis LC-PCR assay. Genomic DNA templates (1 ng) isolated from M. avium subsp. paratuberculosis ATCC 6783 and M. avium ATCC 19421 were amplified, and the amplicons were detected as outlined in Materials and Methods. The large graph shows F57 sequence amplification monitored by LC-Red 705 detection, and the inset shows IC amplification monitored by LC-Red 640 detection. In each reaction, the IC control template was included, and its amplification was confirmed (inset). MAP, M. avium subsp. paratuberculosis.

Article Snippet: a Replicates containing different amounts (140 fg to 10 fg) of M. avium subsp. paratuberculosis genomic DNA isolated from a pure culture of the M. avium subsp. paratuberculosis reference strain (ATCC 6783) were subjected to LC-PCR amplification as outlined in Materials and Methods.

Techniques: Isolation, Sequencing, Control

Journal: Applied and Environmental Microbiology

Article Title: Development of an F57 Sequence-Based Real-Time PCR Assay for Detection of Mycobacterium avium subsp. paratuberculosis in Milk

doi: 10.1128/aem.71.10.5957-5968.2005

Figure Lengend Snippet: FIG. 3. Influence of nonspecific DNA background on amplification of the F57 sequence by the LC-PCR assay. Tenfold serial dilutions of F57 sequence standard dilutions (2 102 to 2 106) and the IC template (2,000 copies) were amplified alone (large graph) or after they were spiked into reaction mixtures containing 200 ng of nonspecific DNA (inset). The inset shows the influence of a DNA background mixture consisting of DNA templates from the following non-M. avium subsp. paratuberculosis mycobacteria: M. avium, M. bovis, M. intracellulare, M. phlei, M. scrofulaceum, M. terrae, and Mycobacterium strain 2333. MAP, M. avium subsp. paratuberculosis.

Article Snippet: a Replicates containing different amounts (140 fg to 10 fg) of M. avium subsp. paratuberculosis genomic DNA isolated from a pure culture of the M. avium subsp. paratuberculosis reference strain (ATCC 6783) were subjected to LC-PCR amplification as outlined in Materials and Methods.

Techniques: Sequencing

Journal: Applied and Environmental Microbiology

Article Title: Development of an F57 Sequence-Based Real-Time PCR Assay for Detection of Mycobacterium avium subsp. paratuberculosis in Milk

doi: 10.1128/aem.71.10.5957-5968.2005

Figure Lengend Snippet: FIG. 4. Quantitative estimation of M. avium subsp. paratuberculosis target copy numbers in defined amounts of purified M. avium subsp. paratuberculosis genomic DNA using the LC-PCR assay. F57 sequence-based quantification standards (2 101 to 2 106 copies) and quasi-unknown samples A and B containing 15 pg and 1.5 pg of M. avium subsp. paratuberculosis DNA, respectively, were amplified. Based on theoretical calculations, samples A and B contained about 3,000 and 300 M. avium subsp. paratuberculosis genome copies, respectively. The target copy numbers in these samples were estimated from the standard curve generated from amplification of the F57 standards. The results obtained from these quantitative assays are summarized in Table 4. MAP, M. avium subsp. paratuberculosis.

Article Snippet: a Replicates containing different amounts (140 fg to 10 fg) of M. avium subsp. paratuberculosis genomic DNA isolated from a pure culture of the M. avium subsp. paratuberculosis reference strain (ATCC 6783) were subjected to LC-PCR amplification as outlined in Materials and Methods.

Techniques: Sequencing, Generated

Journal: Animal Reproduction

Article Title: Detection of Mycobacterium avium subsp. paratuberculosis in reproductive tissue and semen of naturally infected rams

doi: 10.21451/1984-3143-AR2018-0147

Figure Lengend Snippet: DNA amplification by nPCR in semen samples from infected rams with PTB in agarose gel electrophoresis (1.5%). Lanes: M: molecular marker 50 pb; P: positive control (MAP DNA MAP ATCC #700535); N: negative DNA control of ram semen; DNA samples from semen from animals included in the experiment: 1 (positive), 2 (negative), 3 (positive), 4 (negative), 5 (positive), 6 and 7 (negative).

Article Snippet: DNA was obtained from a MAP strain (ATCC #700535) as positive control.

Techniques: DNA Amplification, Infection, Agarose Gel Electrophoresis, Marker, Positive Control, Control